Light microscopy - Methods and protocols

Principles of light microscopy and its use in dermatology

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Condenser-free contrast methods for transmitted-light microscopy

Phase contrast microscopy allows the study of highly transparent yet detail-rich specimens by producing intensity contrast from phase objects within the sample. Presented here is a generalized phase contrast illumination schema in which condenser optics are entirely abrogated, yielding a condenser-free yet highly effective method of obtaining phase contrast in transmitted-light microscopy. A ri...

Effect of Three Light Curing Protocols and Load Cycling on Microleakage of Class V Composite Restorations

  Objective: Different methods have been suggested to overcome the polymerization shrinkage of composite restorations. Changing the light curing protocol to improve polymerization by using new light curing units is among these methods. The new devices are more efficient, portable and durable and produce less heat. This study aimed to assess the marginal microleakage of class V composite restora...

microscopy and analysis Correlative Light and Electron Microscopy

Advanced Light Microscopy Techniques

This article summarizes the basic principles of light microscopy, with examples of applications in biomedicine that illustrate the capabilities of the technique. Handbook of instrumental techniques from CCiTUB Advanced Light Microscopy Techniques 1 BT.4